HomeStore

AmScope 50X-1000X Darkfield Polarizing Metallurgical Microscope + 9MP Camera

AmScope 50X-1000X Darkfield Polarizing Metallurgical Microscope + 9MP Camera

The ME520T is a highly versatile microscope for materials inspection using transmitted and reflected light. Primarily designed as an upright metallurgical microscope, the high-power 100W epi-illumination system can be used for brightfield, darkfield, and basic polarized-light. Wafer inspection and metallographic applications will greatly benefit from the illumination modes. The addition of transmitted lighting provides even more flexibility for biological specimens, or backlighting hollow or porous materials. This instrument will be indispensable in any biology, medical, or materials lab.


Viewing Head


The microscope's head is designed for precision and comfort. The super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. Compensation-free Siedentopf interpupillary adjustment allows any user to properly align the oculars without readjusting focus. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The photo port is equipped with a 23mm tube for attaching cameras with compensating lenses, and is height-adjustable to match focus with the eyepieces. The port is compatible with our YX series C-mount adapters which can attach directly to C-mount cameras without a compensating lens – ideal for 1” format sensors. The head is set to a 30° incline which is ideal for viewing from a standing position, and it can be rotated 360°.

Optics

The quintuple objective turret has three RMS objective mounts and two M25 mounts for BD brightfield/darkfield objective lenses. Five RMS lenses are included for transmitted or reflected lighting ranging from 5X to 100X. 10X and 20X BD lenses are included for use with brightfield and darkfield reflected lighting for extreme versatility. With the 10X eyepieces, six unique magnifications are available from 50X to 1000X. The turret's reversed orientation provides more working room above the stage. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. This is important to guarantee optical resolution and field-flatness when using the episcopic illuminator and any additional filtering.

The Stage

The 3-layer mechanical stage provides smooth and precise movement for examination of specimen slides, with a large translation range for viewing larger samples. The stage's low-position controls are conveniently placed near the coaxial focus knobs for a streamlined workflow. Examining specimens is intuitive and precise for users at any skill level. Symmetrical focus controls are coaxially mounted for fast, intuitive focusing. A height limit can be set to prevent contact damage, as well as presetting optimal focus distance.

Koehler Episcopic Illumination

The microscope is equipped with 10X and 20X BD (brightfield/darkfield) objective lenses which double as darkfield condensers when using reflected lighting. A slider on the front of the illuminator lets you quickly switch between brightfield and darkfield modes, allowing you to highlight different details.

The illuminator is also equipped with a polarizer and analyzer for even more flexibility. Reflected polarized-lighting will pronounce details in a range of materials and microelectronics, particularly those with birefringent components.

The episcopic illuminator provides 100W of incandescent light, with field and aperture diaphragms for precise control of contrast. Proper adjustment will produce even illumination across the field with improved collimation. The optimized brightness and centering will result in consistent lighting for brightfield applications, and critical improvements in darkfield applications.

Koehler Transmitted Illumination


Built on Koehler illumination principals, the sub-stage lighting provides evenly-distributed light for optimal contrast. The rear-mounted 30W halogen light-source provides 100 CRI illumination with ample brightness for contrast-enhancement methods like darkfield. The lower color temperature of the halogen light-source causes less eyestrain compared to high-Kelvin LED. The light condenser uses a swing mechanism for optimized lighting at both high and low magnifications, with a NA0.25 setting for full coverage at extremely low magnifications. An iris diaphragm allows you to adjust the aperture to optimize contrast and depth-of-field. The condenser holder uses rack-and-pinion height adjustment for precision control, and is centerable for proper alignment. The condenser is top-mounted for easier replacement when working with additional condensers. The illuminator's field lens provides an iris diaphragm to control light-spread, optimizing contrast and definition in specimen features.

Digital Imaging

Monitor and capture photos and videos with the included 9MP USB2.0 camera. The C-mount camera is specially designed for use with microscopes, allowing you to watch live images, and to record photos or videos on your computer. This ability to view microscopic images on your computer reduces eye-strain, and allows groups of people to view images at the same time. A 0.5X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

Optical System Infinite-conjugate
Reference Focal Length 200mm
Head Trinocular, 30° incline, 360° rotatable
Interpupillary Adjustment Siedentopf compensation-free gemel, 48-75mm
Ocular Diameter 30mm
Ocular Diopters left ocular
Eyepieces 10X eyepiece with reticle
10X
Photo Port height-adjustable YX series, 23mm tube mount
Objective Lenses Infinity-corrected
Objective Parfocal Distance 45mm
Objective Mounting Thread 3 x RMS 20.32mm, 2 x M25
Objective Turret Reverse quintuple, ball-bearing movement
Focusing System Coaxial coarse and fine focus, tension control
Focus Range
Travel 24mm
Coarse Stroke 37.7mm per rotation
Fine Stroke 0.1mm per rotation
Stage Design Three-layer mechanical stage with caliper
Stage Dimensions 186mm x 138mm
X-Y Translation Range 74mm x 50mm
Transmitted Illumination halogen, continuous-adjustment, rear-mount housing with aspherical collector
Episcopic Illumination 100W halogen, continuous-adjustment, rear-mount housing with aspherical collector
Condenser NA0.9/0.25 achromatic swing condenser with iris diaphragm
Sub-stage Condenser-holder rack and pinion
Power 100-240VAC 50/60Hz wide-band

Objective Lenses

This microscope includes seven objective lenses for metallurgical and biological applications. Two lenses are designated as BD, which can be used for reflected-light darkfield.


Brightfield Metallurgical Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness Mount
5X Plan-achromatic 0.12 15.5mm RMS
50X Plan-achromatic 0.75 0.32mm 0 RMS
100X Plan-achromatic 0.80 2mm 0 RMS

Brightfield/Darkfield Metallurgical Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness Mount
10X Plan-achromatic 0.25 10mm 0 M25
20X Plan-achromatic 0.40 4.3mm 0 M25

Transmitted-light Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness Mount
40X Plan-achromatic 0.65 0.54mm 0.17mm RMS
100X Plan-achromatic 1.25 0.13mm 0.17mm RMS

Camera Specifications
Sensor Type CMOS
Sensor Optical Format 1/2.4"
Active Pixels 9M (3488 x 2616)
Pixel Size 1.67µm x 1.67µm
Active Sensor Area 5.82mm x 4.37mm
Shutter electronic rolling shutter
Sensitivity 0.31V/Lux-sec
Spectral Response 380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate 1.9fps @ 3488x2616
8fps @ 1744x1308
27fps @ 872x654
Connectivity USB 2.0
Lens-mount C-mount
Compensating Lens 0.5X
Power 5VDC over USB

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One trinocular microscope head
  • One microscope body
  • One episcopic illuminator
  • Two light housings
  • Three brightfield metallurgical objective lenses: 5X, 50X, 100X
  • Two brightfield/darkfield metallurgical objective lenses: 10X, 20X
  • Two brightfield transmitted-light objective lenses: 40X, 100X
  • One pair 10X eyepieces
  • One set polarizer and analyzer
  • Two lamp bulbs
  • Two spare fuses
  • One dust cover
  • One sample immersion oil
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB Type B to USB type A cable
  • One software CD
  • $1,369.50

    Original: $4,564.99

    -70%
    AmScope 50X-1000X Darkfield Polarizing Metallurgical Microscope + 9MP Camera

    $4,564.99

    $1,369.50
    Product image 1
    Product image 2
    Product image 3
    Product image 4
    Product image 5
    Product image 6
    Product image 7

    Description

    The ME520T is a highly versatile microscope for materials inspection using transmitted and reflected light. Primarily designed as an upright metallurgical microscope, the high-power 100W epi-illumination system can be used for brightfield, darkfield, and basic polarized-light. Wafer inspection and metallographic applications will greatly benefit from the illumination modes. The addition of transmitted lighting provides even more flexibility for biological specimens, or backlighting hollow or porous materials. This instrument will be indispensable in any biology, medical, or materials lab.


    Viewing Head


    The microscope's head is designed for precision and comfort. The super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. Compensation-free Siedentopf interpupillary adjustment allows any user to properly align the oculars without readjusting focus. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The photo port is equipped with a 23mm tube for attaching cameras with compensating lenses, and is height-adjustable to match focus with the eyepieces. The port is compatible with our YX series C-mount adapters which can attach directly to C-mount cameras without a compensating lens – ideal for 1” format sensors. The head is set to a 30° incline which is ideal for viewing from a standing position, and it can be rotated 360°.

    Optics

    The quintuple objective turret has three RMS objective mounts and two M25 mounts for BD brightfield/darkfield objective lenses. Five RMS lenses are included for transmitted or reflected lighting ranging from 5X to 100X. 10X and 20X BD lenses are included for use with brightfield and darkfield reflected lighting for extreme versatility. With the 10X eyepieces, six unique magnifications are available from 50X to 1000X. The turret's reversed orientation provides more working room above the stage. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

    The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. This is important to guarantee optical resolution and field-flatness when using the episcopic illuminator and any additional filtering.

    The Stage

    The 3-layer mechanical stage provides smooth and precise movement for examination of specimen slides, with a large translation range for viewing larger samples. The stage's low-position controls are conveniently placed near the coaxial focus knobs for a streamlined workflow. Examining specimens is intuitive and precise for users at any skill level. Symmetrical focus controls are coaxially mounted for fast, intuitive focusing. A height limit can be set to prevent contact damage, as well as presetting optimal focus distance.

    Koehler Episcopic Illumination

    The microscope is equipped with 10X and 20X BD (brightfield/darkfield) objective lenses which double as darkfield condensers when using reflected lighting. A slider on the front of the illuminator lets you quickly switch between brightfield and darkfield modes, allowing you to highlight different details.

    The illuminator is also equipped with a polarizer and analyzer for even more flexibility. Reflected polarized-lighting will pronounce details in a range of materials and microelectronics, particularly those with birefringent components.

    The episcopic illuminator provides 100W of incandescent light, with field and aperture diaphragms for precise control of contrast. Proper adjustment will produce even illumination across the field with improved collimation. The optimized brightness and centering will result in consistent lighting for brightfield applications, and critical improvements in darkfield applications.

    Koehler Transmitted Illumination


    Built on Koehler illumination principals, the sub-stage lighting provides evenly-distributed light for optimal contrast. The rear-mounted 30W halogen light-source provides 100 CRI illumination with ample brightness for contrast-enhancement methods like darkfield. The lower color temperature of the halogen light-source causes less eyestrain compared to high-Kelvin LED. The light condenser uses a swing mechanism for optimized lighting at both high and low magnifications, with a NA0.25 setting for full coverage at extremely low magnifications. An iris diaphragm allows you to adjust the aperture to optimize contrast and depth-of-field. The condenser holder uses rack-and-pinion height adjustment for precision control, and is centerable for proper alignment. The condenser is top-mounted for easier replacement when working with additional condensers. The illuminator's field lens provides an iris diaphragm to control light-spread, optimizing contrast and definition in specimen features.

    Digital Imaging

    Monitor and capture photos and videos with the included 9MP USB2.0 camera. The C-mount camera is specially designed for use with microscopes, allowing you to watch live images, and to record photos or videos on your computer. This ability to view microscopic images on your computer reduces eye-strain, and allows groups of people to view images at the same time. A 0.5X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

    Specifications

    Optical System Infinite-conjugate
    Reference Focal Length 200mm
    Head Trinocular, 30° incline, 360° rotatable
    Interpupillary Adjustment Siedentopf compensation-free gemel, 48-75mm
    Ocular Diameter 30mm
    Ocular Diopters left ocular
    Eyepieces 10X eyepiece with reticle
    10X
    Photo Port height-adjustable YX series, 23mm tube mount
    Objective Lenses Infinity-corrected
    Objective Parfocal Distance 45mm
    Objective Mounting Thread 3 x RMS 20.32mm, 2 x M25
    Objective Turret Reverse quintuple, ball-bearing movement
    Focusing System Coaxial coarse and fine focus, tension control
    Focus Range
    Travel 24mm
    Coarse Stroke 37.7mm per rotation
    Fine Stroke 0.1mm per rotation
    Stage Design Three-layer mechanical stage with caliper
    Stage Dimensions 186mm x 138mm
    X-Y Translation Range 74mm x 50mm
    Transmitted Illumination halogen, continuous-adjustment, rear-mount housing with aspherical collector
    Episcopic Illumination 100W halogen, continuous-adjustment, rear-mount housing with aspherical collector
    Condenser NA0.9/0.25 achromatic swing condenser with iris diaphragm
    Sub-stage Condenser-holder rack and pinion
    Power 100-240VAC 50/60Hz wide-band

    Objective Lenses

    This microscope includes seven objective lenses for metallurgical and biological applications. Two lenses are designated as BD, which can be used for reflected-light darkfield.


    Brightfield Metallurgical Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness Mount
    5X Plan-achromatic 0.12 15.5mm RMS
    50X Plan-achromatic 0.75 0.32mm 0 RMS
    100X Plan-achromatic 0.80 2mm 0 RMS

    Brightfield/Darkfield Metallurgical Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness Mount
    10X Plan-achromatic 0.25 10mm 0 M25
    20X Plan-achromatic 0.40 4.3mm 0 M25

    Transmitted-light Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness Mount
    40X Plan-achromatic 0.65 0.54mm 0.17mm RMS
    100X Plan-achromatic 1.25 0.13mm 0.17mm RMS

    Camera Specifications
    Sensor Type CMOS
    Sensor Optical Format 1/2.4"
    Active Pixels 9M (3488 x 2616)
    Pixel Size 1.67µm x 1.67µm
    Active Sensor Area 5.82mm x 4.37mm
    Shutter electronic rolling shutter
    Sensitivity 0.31V/Lux-sec
    Spectral Response 380-650nm with IR-cut filter
    Capture Resolution and Maximum Framerate 1.9fps @ 3488x2616
    8fps @ 1744x1308
    27fps @ 872x654
    Connectivity USB 2.0
    Lens-mount C-mount
    Compensating Lens 0.5X
    Power 5VDC over USB

    Software

    OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
    Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
    Features
    Windows Version
    Live video preview
    Captures still images and video
    Time-lapse capture
    Exposure and white-balance controls
    Dark-field correction
    Digital binning
    Image processing
    Measurement tools, including lines, arcs, polygons
    Segmentation and counting tools
    Image-stitching, extended depth-of-field, HDR
    Fluorescence color-mapping
    Mac/Linux Versions
    Live video preview
    Captures still images and video
    Exposure control
    Color adjustment
    Measurement tools, including lines, arcs, polygons

    Packing List:

  • One trinocular microscope head
  • One microscope body
  • One episcopic illuminator
  • Two light housings
  • Three brightfield metallurgical objective lenses: 5X, 50X, 100X
  • Two brightfield/darkfield metallurgical objective lenses: 10X, 20X
  • Two brightfield transmitted-light objective lenses: 40X, 100X
  • One pair 10X eyepieces
  • One set polarizer and analyzer
  • Two lamp bulbs
  • Two spare fuses
  • One dust cover
  • One sample immersion oil
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB Type B to USB type A cable
  • One software CD