The ME520T is a highly versatile microscope for materials inspection using transmitted and reflected light. Primarily designed as an upright metallurgical microscope, the high-power 100W epi-illumination system can be used for brightfield, darkfield, and basic polarized-light. Wafer inspection and metallographic applications will greatly benefit from the illumination modes. The addition of transmitted lighting provides even more flexibility for biological specimens, or backlighting hollow or porous materials. This instrument will be indispensable in any biology, medical, or materials lab.

Viewing Head

The microscope's head is designed for precision and comfort. The super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. Compensation-free Siedentopf interpupillary adjustment allows any user to properly align the oculars without readjusting focus. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The photo port is equipped with a 23mm tube for attaching cameras with compensating lenses, and is height-adjustable to match focus with the eyepieces. The port is compatible with our YX series C-mount adapters which can attach directly to C-mount cameras without a compensating lens – ideal for 1” format sensors. The head is set to a 30° incline which is ideal for viewing from a standing position, and it can be rotated 360°.

Optics

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. This is important to guarantee optical resolution and field-flatness when using the episcopic illuminator and any additional filtering.

The quintuple objective turret has three RMS objective mounts and two M25 mounts for BD brightfield/darkfield objective lenses. Five RMS lenses are included for transmitted or reflected lighting ranging from 5X to 100X. 10X and 20X BD lenses are included for use with brightfield and darkfield reflected lighting for extreme versatility. With the 10X and 25X eyepieces, ten unique magnifications are available from 50X to 2500X. The turret's reversed orientation provides more working room above the stage. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The 3-layer mechanical stage provides smooth and precise movement for examination of specimen slides, with a large translation range for viewing larger samples. The stage's low-position controls are conveniently placed near the coaxial focus knobs for a streamlined workflow. Examining specimens is intuitive and precise for users at any skill level. Symmetrical focus controls are coaxially mounted for fast, intuitive focusing. A height limit can be set to prevent contact damage, as well as presetting optimal focus distance.

Koehler Episcopic Illumination

The episcopic illuminator provides 100W of incandescent light, with field and aperture diaphragms for precise control of contrast. Proper adjustment will produce even illumination across the field with improved collimation. The optimized brightness and centering will result in consistent lighting for brightfield applications, and critical improvements in darkfield applications.

The microscope is equipped with 10X and 20X BD (brightfield/darkfield) objective lenses which double as darkfield condensers when using reflected lighting. A slider on the front of the illuminator lets you quickly switch between brightfield and darkfield modes, allowing you to highlight different details.

The illuminator is also equipped with a polarizer and analyzer for even more flexibility. Reflected polarized-lighting will pronounce details in a range of materials and microelectronics, particularly those with birefringent components.

Koehler Transmitted Illumination

Built on Koehler illumination principals, the sub-stage lighting provides evenly-distributed light for optimal contrast. The rear-mounted 30W halogen light-source provides 100 CRI illumination with ample brightness for contrast-enhancement methods like darkfield. The lower color temperature of the halogen light-source causes less eyestrain compared to high-Kelvin LED. The light condenser uses a swing mechanism for optimized lighting at both high and low magnifications, with a NA0.25 setting for full coverage at extremely low magnifications. An iris diaphragm allows you to adjust the aperture to optimize contrast and depth-of-field. The condenser holder uses rack-and-pinion height adjustment for precision control, and is centerable for proper alignment. The condenser is top-mounted for easier replacement when working with additional condensers. The illuminator's field lens provides an iris diaphragm to control light-spread, optimizing contrast and definition in specimen features.

Digital Imaging

Monitor and capture photos and videos with the included 18.0MP USB3.0 camera. The C-mount camera is specially designed for use with microscopes, allowing you to watch live images, and to record photos or videos on your computer. This ability to view microscopic images on your computer reduces eye-strain, and allows groups of people to view images at the same time. The USB 3.0 connection provides greater bandwidth for faster frame-rates. A 0.5X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

Optical System

Infinite-conjugate

Reference Focal Length

200mm

Head

Trinocular, 30° incline, 360° rotatable

Interpupillary Adjustment

Siedentopf compensation-free gemel, 48-75mm

Ocular Diameter

30mm

Ocular Diopters

left ocular

Eyepieces

10X eyepiece with reticle
10X, 25X

Photo Port

height-adjustable YX series, 23mm tube mount

Objective Lenses

Infinity-corrected

Objective Parfocal Distance

45mm

Objective Mounting Thread

3 x RMS 20.32mm, 2 x M25

Objective Turret

Reverse quintuple, ball-bearing movement

Focusing System

Coaxial coarse and fine focus, tension control

Focus Range

Travel	24mm
Coarse Stroke	37.7mm per rotation
Fine Stroke	0.1mm per rotation

Stage Design

Three-layer mechanical stage with caliper

Stage Dimensions

186mm x 138mm

X-Y Translation Range

74mm x 50mm

Transmitted Illumination

halogen, continuous-adjustment, rear-mount housing with aspherical collector

Episcopic Illumination

100W halogen, continuous-adjustment, rear-mount housing with aspherical collector

Condenser

NA0.9/0.25 achromatic swing condenser with iris diaphragm

Sub-stage Condenser-holder

rack and pinion

Power

100-240VAC 50/60Hz wide-band

Objective Lenses

This microscope includes seven objective lenses for metallurgical and biological applications. Two lenses are designated as BD, which can be used for reflected-light darkfield.

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness	Mount
Brightfield Metallurgical Objective Lenses

5X	Plan-achromatic	0.12	15.5mm	—	RMS
50X	Plan-achromatic	0.75	0.32mm	0	RMS
100X	Plan-achromatic	0.80	2mm	0	RMS

Brightfield/Darkfield Metallurgical Objective Lenses

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness	Mount
10X	Plan-achromatic	0.25	10mm	0	M25
20X	Plan-achromatic	0.40	4.3mm	0	M25

Transmitted-light Objective Lenses

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness	Mount
40X	Plan-achromatic	0.65	0.54mm	0.17mm	RMS
100X	Plan-achromatic	1.25	0.13mm	0.17mm	RMS

Camera Specifications

Sensor Type	CMOS

Sensor Optical Format	1/2.3"

Active Pixels	18M (4912 x 3684)

Pixel Size	1.25µm x 1.25µm

Active Sensor Area	6.14mm x 4.61mm

Shutter	electronic rolling shutter

Spectral Response	380-650nm with IR-cut filter

Capture Resolution and Maximum Framerate	5.6fps @ 4912x3684 18.1fps @ 2456x1842 32.2fps @ 1228x922

Connectivity	USB 3.0

Lens-mount	C-mount

Compensating Lens	0.5X

Power	5VDC over USB

Software

OS Requirements

Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+

Hardware Requirements

Intel Core2 2.8GHz or comparable processor, 4GB RAM

Features

Windows Version

Live video preview

Captures still images and video

Time-lapse capture

Exposure and white-balance controls

Dark-field correction

Digital binning

Image processing

Measurement tools, including lines, arcs, polygons

Segmentation and counting tools

Image-stitching, extended depth-of-field, HDR

Fluorescence color-mapping

Mac/Linux Versions

Live video preview

Captures still images and video

Exposure control

Color adjustment

Measurement tools, including lines, arcs, polygons

Packing List:

One trinocular microscope head
One microscope body
One episcopic illuminator
Two light housings
Three brightfield metallurgical objective lenses: 5X, 50X, 100X
Two brightfield/darkfield metallurgical objective lenses: 10X, 20X
Two brightfield transmitted-light objective lenses: 40X, 100X
One pair 10X eyepieces
One pair 25X eyepieces
One set polarizer and analyzer
Two lamp bulbs
Two spare fuses
One dust cover
One sample immersion oil
One C-mount USB camera
One 0.5X reduction lens
Two sizing adapters
One USB 3.0 Type B to type A cable
One software CD

Viewing Head

Optics

The Stage

Koehler Episcopic Illumination

Koehler Transmitted Illumination

Digital Imaging

Specifications

Objective Lenses

Brightfield Metallurgical Objective Lenses

Brightfield/Darkfield Metallurgical Objective Lenses

Transmitted-light Objective Lenses

Software

Packing List:

18.0 M.P.

AmScope 50X-2500X Darkfield Polarizing Metallurgical Microscope + 18MP Camera